We guarantee a minimum hatch-out of 255,000 nauplii (baby brine shrimp) per gram of cysts following a 24 hour incubation period. Based on the most recent harvest, this equates to an average hatch-out of 90%. Feel confident purchasing brine shrimp eggs from Reef2Land. We will return your money if you do not achieve the specified hatching results from artemia cysts that have been properly stored and handled. Eggs are thoroughly disinfected in a hypochlorite solution during processing. Inventory is periodically tested, stored in a temperature-controlled (40 degrees F) warehouse, and packaged on demand.
Instructions and Guidelines:
Below you will find complete and simple-to-follow brine shrimp hatching instructions!
Storing Brine Shrimp Eggs - At Reef2Land we know Brine Shrimp Eggs
First of all, you need to start with healthy, properly stored eggs from a reputable source. Any brine shrimp eggs you buy should be stored as follows.
In a tightly sealed container; free from moisture; and in a cool environment at or below 50°F. (Refrigeration is ideal for short term storage, i.e., less than three to four weeks; for longer term storage, eggs are best kept at or below freezing.)
When you receive your Elite Brine Shrimp Eggs:
Upon receiving your eggs, divide them into an amount that will be consumed within three to four weeks and store this amount in a tightly sealed container in the refrigerator. The remainder should be stored, in a tightly sealed container, in the freezer. Also, make sure to keep your eggs away from any direct light source. This is why every order we ship is sent in an air tight amber plastic jar with a lid liner free of charge.
Keep in mind that freezing can lower metabolic activity and delay hatch-out. We suggest removing eggs from the freezer one day prior to getting ready to hatch.
Hatching Environment: What we recommend at Reef2Land for the best results (must follow for Guarantee):
25 parts per thousand (ppt) salt solution, or approximately 1 and 2/3 tablespoons of salt per quart of water. This equates to around 1.018 specific gravity as measured with a hydrometer. Be sure to use marine salt or solar salt.
Proper pH is important in hatching brine shrimp. A starting pH of 8.0 or higher is recommended. In areas where the water pH is below 7, Epson salt or magnesium sulfate can be added at the rate of 1/2 teaspoon per quart of solution to buffer the hatching solution.
Optimum water temperature for a 24-hour complete hatch is 80-82°F or 26-28°C. Lowering the temperature can result in a longer hatching time.
Illumination is necessary to initiate hatching within the embryo during the first few hours of incubation. Maintaining a light source during the entire incubation period is recommended to obtain optimum hatch results and for temperature control.
Constant aeration is necessary to keep cysts in suspension and to provide sufficient oxygen levels for the cysts to hatch. A minimum of 3 parts per million dissolved oxygen during the incubation is recommended. Strong aeration should not damage or hurt the brine shrimp cysts or nauplii.
1 gram per liter or quart or approximately 1/2 level teaspoon of cysts per quart is recommended. A higher stocking density will result in a lower hatch percentage.
Flat-bottom hatching vessels should be avoided. Cone or "V" bottomed containers are best to insure that the cysts remain in suspension during hatching. Be sure to thoroughly wash the hatching cone with a light chlorine solution, rinse, and allow to air-dry between uses. Avoid soap. Soap will leave a slight residue which will foam from aeration during hatching and leave cysts stranded above the water level.
Generally, the optimum incubation time is 24 hours. Egg which has been properly stored for more than 2-3 months may require additional incubation time — up to 30-36 hours. The majority of the time eggs will hatch in as little as 18 hours. If a smaller size nauplii is desired, a harvest time of 18 hours is recommended.
Brine shrimp egg is sometimes very buoyant. In order to maximize the hatching percentage, it is sometimes helpful to swirl the water inside the hatching container with your finger once or twice at intervals in the first 4 to 6 hours of incubation in order to knock down eggs that have been stranded on the side of the container above the water-line. After about 6 hours, the eggs are usually well-hydrated and will stay in the water column.
Hatching Procedure: The following steps will achieve optimum brine shrimp hatch rates.
Place hatching cone or similarly shaped vessel in well-lit area. Cone should be semi-translucent for ease of harvesting and light transmission.
Fill cone with water and adjust salinity to 25 ppt (parts per thousand). Optimum hatching temperature is 82°F (28°C).
What to do after your Brine Shrimp Eggs hatch:
After your brine shrimp eggs hatch, turn off & remove aeration then wait several minutes for the shells and and baby brine shrimp to separate. Newly hatched nauplii will settle to the bottom of the container or move towards a light source, the shells will then float to the surface. Once separated, your nauplii can be siphoned from the bottom with someaquarium airline tubing or gently drained through the bottom of the container through a valve, if you have one.
Remember to rinse:
The warm temperatures from the hatching creatse ideal conditions for a bacteria blooms. Rinsing the baby brine shrimp in a fine mesh net or sieve using clean fresh or salt water is important before feeding them to your fish to avoid contamination.
Clean and store your equipment for their next use:
Tanks and brine shrimp hatching equipment should be cleaned and disinfected regularly to prevent cross contamination between batches.